Finding primers specific to your PCR template (using Primer3 and BLAST).
PCR Template
(A refseq record is preferred) Help
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From To
 
Primer Parameters
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Exon/intron selection
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Note: Parameter values that differ from the default are highlighted in yellow
Primer Pair Specificity Checking Parameters
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Clear

Or, upload file:
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Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type. Help

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Primer must have at least total mismatches to unintended targets, including
at least mismatches within the last bps at the 3' end. Help
Ignore targets that have or more mismatches to the primer. Help
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Note: Parameter values that differ from the default are highlighted in yellow