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J Biol Chem. 1998 Mar 13;273(11):6139-43.

Isolation from an ant Myrmecia gulosa of two inducible O-glycosylated proline-rich antibacterial peptides.

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1
School of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia. jmackint@rna.bio.mq.edu.au

Abstract

Reported here is the isolation and characterization of two antibacterial peptides synthesized in an ant Myrmecia gulosa in response to bacterial challenge. The peptides were purified by reversed-phase high performance liquid chromatography and characterized by peptide sequencing and mass spectrometry. Both peptides were formed from 16 amino acids, were rich in proline ( approximately 30%), and had N-acetylgalactosamine O-linked to a conserved threonine. The activity of a synthetic non-glycosylated isoform was markedly reduced demonstrating that glycosylation was necessary for maximum activity. The peptides were active only against growing Escherichia coli. They were inactive against stationary cells, Gram-positive bacteria, the yeast Candida albicans, two species of mammalian cells, and bovine pestivirus.

PMID:
9497332
[Indexed for MEDLINE]
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