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Sample GSM93731 Query DataSets for GSM93731
Status Public on Jan 27, 2006
Title M8L
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, limbus
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 962.9 P 0.000581
AFFX-BioB-M_at 1386.3 P 0.000044
AFFX-BioB-3_at 927.4 P 0.00006
AFFX-BioC-5_at 2173.1 P 0.00006
AFFX-BioC-3_at 1548.8 P 0.000044
AFFX-BioDn-5_at 2170.7 P 0.000044
AFFX-BioDn-3_at 6836.3 P 0.000052
AFFX-CreX-5_at 30473 P 0.000052
AFFX-CreX-3_at 27659 P 0.000044
AFFX-DapX-5_at 42.4 A 0.686292
AFFX-DapX-M_at 46.2 A 0.686277
AFFX-DapX-3_at 8.5 A 0.966335
AFFX-LysX-5_at 61.5 A 0.274972
AFFX-LysX-M_at 10.4 A 0.957038
AFFX-LysX-3_at 42.4 A 0.396911
AFFX-PheX-5_at 9.8 A 0.876428
AFFX-PheX-M_at 61.9 A 0.672921
AFFX-PheX-3_at 56 A 0.455413
AFFX-ThrX-5_at 10.1 A 0.989683
AFFX-ThrX-M_at 10 A 0.617401

Total number of rows: 45101

Table truncated, full table size 1221 Kbytes.




Supplementary file Size Download File type/resource
GSM93731.CEL.gz 5.4 Mb (ftp)(http) CEL
GSM93731.EXP.gz 368 b (ftp)(http) EXP
Raw data provided as supplementary file

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