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Sample GSM93730 Query DataSets for GSM93730
Status Public on Jan 27, 2006
Title M8C
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, cornea
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1312.9 P 0.00141
AFFX-BioB-M_at 1927.8 P 0.000044
AFFX-BioB-3_at 1851.6 P 0.000195
AFFX-BioC-5_at 3229.7 P 0.000044
AFFX-BioC-3_at 1697.7 P 0.000044
AFFX-BioDn-5_at 2630.5 P 0.000044
AFFX-BioDn-3_at 9448.7 P 0.000044
AFFX-CreX-5_at 37825.3 P 0.000052
AFFX-CreX-3_at 32541.7 P 0.000044
AFFX-DapX-5_at 76 A 0.455413
AFFX-DapX-M_at 9 A 0.932322
AFFX-DapX-3_at 9.7 A 0.957038
AFFX-LysX-5_at 72.7 A 0.287743
AFFX-LysX-M_at 6.7 A 0.984817
AFFX-LysX-3_at 116.4 M 0.062929
AFFX-PheX-5_at 16.5 A 0.960339
AFFX-PheX-M_at 12.2 A 0.937071
AFFX-PheX-3_at 63 A 0.48511
AFFX-ThrX-5_at 17.4 A 0.910522
AFFX-ThrX-M_at 126.6 A 0.250796

Total number of rows: 45101

Table truncated, full table size 1222 Kbytes.




Supplementary file Size Download File type/resource
GSM93730.CEL.gz 6.1 Mb (ftp)(http) CEL
GSM93730.EXP.gz 368 b (ftp)(http) EXP
Raw data provided as supplementary file

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