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Sample GSM93727 Query DataSets for GSM93727
Status Public on Jan 27, 2006
Title M6L
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, limbus
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 5445.9 P 0.002023
AFFX-BioB-M_at 9802.3 P 0.000044
AFFX-BioB-3_at 8268.3 P 0.000044
AFFX-BioC-5_at 14596.3 P 0.000044
AFFX-BioC-3_at 8659.2 P 0.000044
AFFX-BioDn-5_at 15854 P 0.000044
AFFX-BioDn-3_at 41031.3 P 0.000044
AFFX-CreX-5_at 141519.3 P 0.000052
AFFX-CreX-3_at 126063.8 P 0.000044
AFFX-DapX-5_at 136.5 A 0.287743
AFFX-DapX-M_at 47.9 A 0.868639
AFFX-DapX-3_at 19.2 A 0.971543
AFFX-LysX-5_at 214.7 A 0.123572
AFFX-LysX-M_at 29.9 A 0.891021
AFFX-LysX-3_at 165.6 A 0.645547
AFFX-PheX-5_at 198.6 A 0.396911
AFFX-PheX-M_at 39.3 A 0.783496
AFFX-PheX-3_at 38.1 A 0.824672
AFFX-ThrX-5_at 47.9 A 0.910522
AFFX-ThrX-M_at 16.2 A 0.60308

Total number of rows: 45101

Table truncated, full table size 1225 Kbytes.




Supplementary file Size Download File type/resource
GSM93727.CEL.gz 5.9 Mb (ftp)(http) CEL
GSM93727.EXP.gz 368 b (ftp)(http) EXP
Raw data provided as supplementary file

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