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Sample GSM93724 Query DataSets for GSM93724
Status Public on Jan 27, 2006
Title M5L
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, limbus
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 20499.3 P 0.001796
AFFX-BioB-M_at 38500.2 P 0.000044
AFFX-BioB-3_at 39363.4 P 0.000044
AFFX-BioC-5_at 59267.6 P 0.000044
AFFX-BioC-3_at 26561.9 P 0.000044
AFFX-BioDn-5_at 44953.9 P 0.000044
AFFX-BioDn-3_at 157736.9 P 0.000044
AFFX-CreX-5_at 607695.6 P 0.000044
AFFX-CreX-3_at 528005.8 P 0.000044
AFFX-DapX-5_at 310.9 A 0.824672
AFFX-DapX-M_at 39.5 A 0.953518
AFFX-DapX-3_at 39.2 A 0.993813
AFFX-LysX-5_at 40.2 A 0.979978
AFFX-LysX-M_at 383 A 0.574038
AFFX-LysX-3_at 120.5 A 0.824672
AFFX-PheX-5_at 73.7 A 0.987453
AFFX-PheX-M_at 96.4 A 0.957038
AFFX-PheX-3_at 1093.2 A 0.559354
AFFX-ThrX-5_at 72.9 A 0.973991
AFFX-ThrX-M_at 187.3 A 0.824672

Total number of rows: 45101

Table truncated, full table size 1228 Kbytes.




Supplementary file Size Download File type/resource
GSM93724.CEL.gz 5.4 Mb (ftp)(http) CEL
GSM93724.EXP.gz 368 b (ftp)(http) EXP
Raw data provided as supplementary file

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