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Sample GSM93720 Query DataSets for GSM93720
Status Public on Jan 27, 2006
Title M3L
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, limbus
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 12565 P 0.002275
AFFX-BioB-M_at 21476.2 P 0.000044
AFFX-BioB-3_at 23691.6 P 0.000052
AFFX-BioC-5_at 29920 P 0.000044
AFFX-BioC-3_at 15502.8 P 0.000044
AFFX-BioDn-5_at 25995.3 P 0.000044
AFFX-BioDn-3_at 94591.4 P 0.000052
AFFX-CreX-5_at 352021.4 P 0.000044
AFFX-CreX-3_at 275703.5 P 0.000044
AFFX-DapX-5_at 635.7 A 0.529762
AFFX-DapX-M_at 55.5 A 0.997444
AFFX-DapX-3_at 122.7 A 0.824756
AFFX-LysX-5_at 956.9 A 0.313723
AFFX-LysX-M_at 149.9 A 0.941556
AFFX-LysX-3_at 600.7 A 0.440646
AFFX-PheX-5_at 534.3 A 0.559354
AFFX-PheX-M_at 189 A 0.686277
AFFX-PheX-3_at 180.3 A 0.921998
AFFX-ThrX-5_at 184.9 A 0.910522
AFFX-ThrX-M_at 790.7 A 0.574038

Total number of rows: 45101

Table truncated, full table size 1228 Kbytes.




Supplementary file Size Download File type/resource
GSM93720.CEL.gz 5.6 Mb (ftp)(http) CEL
GSM93720.EXP.gz 364 b (ftp)(http) EXP
Raw data provided as supplementary file

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