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Sample GSM93718 Query DataSets for GSM93718
Status Public on Jan 27, 2006
Title M2C
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, cornea
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 22510.6 P 0.000754
AFFX-BioB-M_at 36273.8 P 0.000044
AFFX-BioB-3_at 30563.3 P 0.000044
AFFX-BioC-5_at 50129.4 P 0.000044
AFFX-BioC-3_at 26116.3 P 0.000044
AFFX-BioDn-5_at 53435.9 P 0.000044
AFFX-BioDn-3_at 134498.3 P 0.000044
AFFX-CreX-5_at 449500.6 P 0.000052
AFFX-CreX-3_at 427126.2 P 0.000044
AFFX-DapX-5_at 416.4 A 0.712274
AFFX-DapX-M_at 157.9 A 0.868639
AFFX-DapX-3_at 988.6 A 0.083592
AFFX-LysX-5_at 270.5 A 0.868639
AFFX-LysX-M_at 201.8 A 0.737173
AFFX-LysX-3_at 1499.1 P 0.036569
AFFX-PheX-5_at 121.5 A 0.83416
AFFX-PheX-M_at 630.7 A 0.48511
AFFX-PheX-3_at 1193.6 A 0.078002
AFFX-ThrX-5_at 146.5 A 0.910522
AFFX-ThrX-M_at 863.4 A 0.340648

Total number of rows: 45101

Table truncated, full table size 1228 Kbytes.




Supplementary file Size Download File type/resource
GSM93718.CEL.gz 5.8 Mb (ftp)(http) CEL
GSM93718.EXP.gz 367 b (ftp)(http) EXP
Raw data provided as supplementary file

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