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Sample GSM93717 Query DataSets for GSM93717
Status Public on Jan 27, 2006
Title M1L
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, limbus
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 14159 P 0.000225
AFFX-BioB-M_at 24943.4 P 0.000044
AFFX-BioB-3_at 23577.3 P 0.000044
AFFX-BioC-5_at 34917.3 P 0.000044
AFFX-BioC-3_at 19092.2 P 0.000044
AFFX-BioDn-5_at 35688.6 P 0.000044
AFFX-BioDn-3_at 106488.6 P 0.000044
AFFX-CreX-5_at 362675.9 P 0.000052
AFFX-CreX-3_at 330695.7 P 0.000044
AFFX-DapX-5_at 230 A 0.455409
AFFX-DapX-M_at 339.5 A 0.645547
AFFX-DapX-3_at 66.2 A 0.686277
AFFX-LysX-5_at 51.5 A 0.860518
AFFX-LysX-M_at 179.5 A 0.772364
AFFX-LysX-3_at 1004.2 M 0.062929
AFFX-PheX-5_at 48 A 0.963431
AFFX-PheX-M_at 98 A 0.852061
AFFX-PheX-3_at 499.9 A 0.250777
AFFX-ThrX-5_at 59.6 A 0.957038
AFFX-ThrX-M_at 273.6 A 0.529762

Total number of rows: 45101

Table truncated, full table size 1226 Kbytes.




Supplementary file Size Download File type/resource
GSM93717.CEL.gz 5.6 Mb (ftp)(http) CEL
GSM93717.EXP.gz 362 b (ftp)(http) EXP
Raw data provided as supplementary file

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