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Sample GSM93716 Query DataSets for GSM93716
Status Public on Jan 27, 2006
Title M1C
Sample type RNA
 
Source name anterior eye
Organism Mus musculus
Characteristics Balb/c mouse
Wild type, cornea
Extracted molecule total RNA
Extraction protocol limbal and corneal epithelial basal cells are isolated using laser cupture microdissection (LCM). 300 cells from each location were collected and total RNA were extracted, mRNA were amplified using Arcturus (CA) cRNA amplification kit and the cRNAs were subjected to gene microarray hybridization (Affymetrix).
Label biotin
Label protocol Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type, cornea, limbus, epithelial basal cells
Data processing Affymetrix Gene Operating System 1.2
 
Submission date Jan 25, 2006
Last update date Aug 28, 2018
Contact name Mingyuan Zhou
E-mail(s) m-zhou@northwestern.edu, r-lavker@northwestern.edu
Organization name Northwestern.edu
Department Dermatology
Lab 9-031
Street address 303 E. Chicago Ave.
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE4098 Mouse limbal epithelial basal cells vs. corneal epithelial basal cell gene expression profile
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 14351.6 P 0.000972
AFFX-BioB-M_at 24785.3 P 0.000052
AFFX-BioB-3_at 19095.7 P 0.000052
AFFX-BioC-5_at 33601.3 P 0.000044
AFFX-BioC-3_at 15900.8 P 0.000044
AFFX-BioDn-5_at 33046.2 P 0.000044
AFFX-BioDn-3_at 94253.8 P 0.000044
AFFX-CreX-5_at 339330.4 P 0.000052
AFFX-CreX-3_at 306724.7 P 0.000044
AFFX-DapX-5_at 765.7 A 0.102165
AFFX-DapX-M_at 119 A 0.834139
AFFX-DapX-3_at 399.5 A 0.724854
AFFX-LysX-5_at 845.7 A 0.216524
AFFX-LysX-M_at 534.6 A 0.440646
AFFX-LysX-3_at 1199.8 A 0.067678
AFFX-PheX-5_at 96.2 A 0.814869
AFFX-PheX-M_at 142.4 A 0.814869
AFFX-PheX-3_at 204.6 A 0.852061
AFFX-ThrX-5_at 64.7 A 0.998204
AFFX-ThrX-M_at 561.1 A 0.574038

Total number of rows: 45101

Table truncated, full table size 1228 Kbytes.




Supplementary file Size Download File type/resource
GSM93716.CEL.gz 5.9 Mb (ftp)(http) CEL
GSM93716.EXP.gz 366 b (ftp)(http) EXP
Raw data provided as supplementary file

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