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Sample GSM5575913 Query DataSets for GSM5575913
Status Public on Sep 15, 2021
Title CM/miR-126Δ/Δ-2
Sample type SRA
 
Source name bone morrow
Organism Mus musculus
Characteristics cell type: LSK from mice bone morrow cells
genotype/variation: CM/miR-126delta/delta
treatment: poly(I;C)
Stage: pre-leukemic [6 weeks after poly(I;C) induction]
molecule concentration: 20ng/ul
Extracted molecule total RNA
Extraction protocol Library contruction was used KAPA RNA HyperPrep Kit with RiboErase (HMR) according to manufacturer's protocol.
Depletion of ribosomal RNA (rRNA) and the rapid construction of stranded RNA-Seq libraries
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description Sample_37510_745_S15_Group_C
Data processing Base calling was done using Illumina RTA and bcl2fastq v2.18.0.12
Sequences were aligned to mm10 using Tophat2 with default settings
Gene expression level of Ensembl GRCm38 coding genes were quantified using HTSeq v0.6.1p1
Genome_build: mm10
Supplementary_files_format_and_content: tab delimited text file gene expression counts
 
Submission date Sep 13, 2021
Last update date Sep 15, 2021
Contact name WEI CHEN
E-mail(s) weichen2@coh.org
Organization name City of Hope National Medical Center
Department Integrative Genomics Core
Street address 655 e huntington dr
City monrovia
State/province CA
ZIP/Postal code 91016
Country USA
 
Platform ID GPL17021
Series (1)
GSE184015 Targeting miR-126 in inv(16) acute myeloid leukemia inhibits leukemia development and leukemia stem cell maintenance
Relations
BioSample SAMN21412384
SRA SRX12155379

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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