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Sample GSM5560181 Query DataSets for GSM5560181
Status Public on Oct 20, 2021
Title Wild-type Vehicle
Sample type SRA
 
Source name Lung mesenchymal cells
Organism Mus musculus
Characteristics genotype: Wild-type
tissue: Lung
treatment: Vehicle
Extracted molecule polyA RNA
Extraction protocol Mesenchymal cells were isolated from fresh lung by negative sorting of Cd45, Cd31, Cd326.
Cellular suspensions were loaded on a Chromium Controller instrument (10x Genomics) to generate single-cell Gel-Bead-in-Emulsions (GEMs) and perform single cell partitioning and barcoding.
10x Genomics Single Cell 3' RNA-seq kit v3 User Guide
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Cellranger v3.1 coupled with STAR V2.7.2b for reads alignment and counts summary
Raw data and quality control were processed using R package 'Seurat'.
SCTransform' was used for normalization and variance stabilization
Genome_build: GRCm38 (mm10)
Supplementary_files_format_and_content: raw counts data and normalized data matrix in .gz
 
Submission date Sep 07, 2021
Last update date Oct 20, 2021
Contact name Yong Huang
E-mail(s) yh9fj@virginia.edu
Phone (434) 243-0842
Organization name University of Viginia
Department Medicine
Street address 1340 Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platform ID GPL24247
Series (1)
GSE183545 Monoclonal Antibody Targeting Pear1 for Pulmonary Fibrosis Therapy [scRNA-seq]
Relations
BioSample SAMN21249179
SRA SRX12028842

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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