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Sample GSM4908565 Query DataSets for GSM4908565
Status Public on Oct 15, 2021
Title CB IL-7 + IL-15 n°2
Sample type RNA
 
Source name CB IL-7 + IL-15 n°2
Organism Homo sapiens
Characteristics source: cord blood
cell type: transgenic T cells
culture condition: IL-7 + IL-15
Treatment protocol T cells were transduced with a retroviral vector encoding an HLA-DRB1*04-restricted TCR targeting the human papillomavirus oncoprotein E7, and cultured with IL-2 or with IL-7 and IL-15.
Growth protocol Peripheral and cord blood mononuclear cells were obtained from healthy human donors and cord blood units that were not compliant with banking standards. T cells were activated with anti-CD3/28 microbeads and expanded with IL-2 or with IL-7 and IL-15.
Extracted molecule total RNA
Extraction protocol Ten days after activation, transgenic T cells were lysed and RNA was extracted using Qiagen Rneasy Mini Kit according to manufacturer instructions.
Label NA
Label protocol No label is used in Nanostring
 
Hybridization protocol A hundred nanograms of total RNA were hybridized with the NanoString Technologies nCounter CAR T Characterization panel kit according to the CodeSet Hybridization Setup instructions and the nCounter SPRINT Profiler User Manual.
Scan protocol The nCounter SPRINT Profiler was used to access to Nanostring molecular barcoding technology.
Data processing Gene expression data were analyzed using nSolver Analysis Software (version 4.0) and nCounter Advanced Analysis Software (version 158 2.0.134).
Normalization of the data was done using the algorithm automatically performed by the nCounter Advanced Analysis Software. Only endogenous gene normalzed counts were generated by the software and used for analysis.
 
Submission date Nov 13, 2020
Last update date Oct 15, 2021
Contact name Chrystel Marton
Organization name UMR1098 INSERM
Street address 8 rue du Dr Jean-François-Xavier Girod
City Besançon
ZIP/Postal code 25020
Country France
 
Platform ID GPL29417
Series (1)
GSE161483 Nanostring CAR T Characterization panel comparing expression in cord blood-derived transgenic T cells cultured with IL-2 or with IL-7 and IL-15, peripheral blood-derived transgenic T cells cultured with IL-2

Data table header descriptions
ID_REF
VALUE Normalized signal

Data table
ID_REF VALUE
POS_A
POS_B
POS_C
POS_D
POS_E
POS_F
NEG_A
NEG_B
NEG_C
NEG_D
NEG_E
NEG_F
NEG_G
NEG_H
ACACA 39.0866675302024
ACAD10 163.618608265963
ACADVL 1580.73755430284
ACOT2 83.6272886692702
ACSF2 48.1765902116448
ACSL5 415.409466541919

Total number of rows: 794

Table truncated, full table size 17 Kbytes.




Supplementary file Size Download File type/resource
GSM4908565_CB-IL715-2_10.RCC.gz 8.7 Kb (ftp)(http) RCC
Raw data provided as supplementary file
Processed data included within Sample table

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