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Sample GSM2736343 Query DataSets for GSM2736343
Status Public on Oct 19, 2021
Title NH_FLI_220_brain_RNAseq
Sample type SRA
Source name brain
Organism Danio rerio
Characteristics strain: AB JxTu
tissue: brain
age: 1 year
Sex: male
perturbation: swim-tunnel
Extracted molecule total RNA
Extraction protocol Extraction was done as described in Baumgart et al. 2012. (PMID:22487494)
Library preparation was done using Illumina's TruSeq RNA Library Preparation Kit v2 following the manufacturer's instruction.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Data processing Sequence information was extracted in FastQ format using bcl2fastq software 1.8.4 data processing step.
Reads were mapped using STAR (2.4.1d).
Reads per gene were counted using featureCounts (1.4.6-p4).
Counts were normalized to RPKM values as described in Mortazavi et al. (2008).
Genome_build: genome: GRCz10, and respective gene annotation (GRCz10.84).
Supplementary_files_format_and_content: The Excel files include raw counts (sample_counts.xls) and RPKM values (sample_rpkms.xls) of all genes for each sample.
Submission date Aug 09, 2017
Last update date Oct 19, 2021
Contact name JenAge Project
Organization name Leibniz Institute for Age Research - Fritz Lipmann Institute
Street address Beutenbergstr. 11
City Jena
ZIP/Postal code D-07745
Country Germany
Platform ID GPL18413
Series (1)
GSE102424 Sequencing of Danio rerio brain for 2 age groups after physical exercise
BioSample SAMN07485922
SRA SRX3082014

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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