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Series GSE90476 Query DataSets for GSE90476
Status Public on Dec 01, 2021
Title miRNA-9 regulates axon regeneration through the transcriptional regulator REST and the epigenetic factor UHRF1 [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Injured sensory neurons successfully activate a pro-regenerative transcriptional program to enable axon regeneration and functional recovery, but the roles of genes that are inactivated after injury remain poorly understood. Analysis of the miRNA expression profile triggered by axon injury revealed down-regulation of the neural development associated miRNA, miR-9. A target of miR-9 is the critical epigenetic regulator involved in DNA methylation, ubiquitin-like containing PHD ring finger 1 (UHRF1), which increased after injury to promote axon regeneration. UHRF1 is known to repress the transcription of tumor suppressor genes through DNA methylation and we show that UHRF1 interacts with DNMT1 to methylate the promoter region of the tumor suppressors PTEN and CDKN1A, thereby triggering their inactivation. We also reveal that UHRF1 represses the transcriptional regulator REST. Our findings define an epigenetic mechanism that silences the transcription of axon growth suppressors to promote axon regeneration in sensory neurons.
 
Overall design Examining transcriptional changes after knockdown of UHRF1 in uninjured neurons
 
Contributor(s) Oh YM, Mahar M, Larson D, Ewan EE, Cavalli V
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Submission date Nov 23, 2016
Last update date Dec 02, 2021
Contact name Valeria Cavalli
E-mail(s) cavalli@pcg.wustl.edu
Organization name Washington University in St. Louis
Department Anatomy and Neurobiology
Street address 660 S. Euclid Ave.
City Saint Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM2402622 shControl_Rep1
GSM2402623 shControl_Rep2
GSM2402624 shControl_Rep3
This SubSeries is part of SuperSeries:
GSE90477 Gene repression by DNA methylation promotes axon regeneration
Relations
BioProject PRJNA354789
SRA SRP093837

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90476_RAW.tar 1.2 Mb (http)(custom) TAR (of TXT)
GSE90476_UHRF1_RNAseq_shComparison.xlsx 4.0 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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