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Series GSE90475 Query DataSets for GSE90475
Status Public on Dec 01, 2021
Title miRNA-9 regulates axon regeneration through the transcriptional regulator REST and the epigenetic factor UHRF1 [miRNA array]
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Injured sensory neurons successfully activate a pro-regenerative transcriptional program to enable axon regeneration and functional recovery, but the roles of genes that are inactivated after injury remain poorly understood. Analysis of the miRNA expression profile triggered by axon injury revealed down-regulation of the neural development associated miRNA, miR-9. A target of miR-9 is the critical epigenetic regulator involved in DNA methylation, ubiquitin-like containing PHD ring finger 1 (UHRF1), which increased after injury to promote axon regeneration. UHRF1 is known to repress the transcription of tumor suppressor genes through DNA methylation and we show that UHRF1 interacts with DNMT1 to methylate the promoter region of the tumor suppressors PTEN and CDKN1A, thereby triggering their inactivation. We also reveal that UHRF1 represses the transcriptional regulator REST. Our findings define an epigenetic mechanism that silences the transcription of axon growth suppressors to promote axon regeneration in sensory neurons.
 
Overall design miRNA expression profiling of spot cultures collected at Day in Vitro (DIV) 7, axotomized spot cultures at DIV7 collected at 1 hour, 3 hour, or 8 hours post injury.
 
Contributor(s) Oh YM, Mahar M, Larson D, Ewan EE, Cavalli V
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Submission date Nov 23, 2016
Last update date Dec 02, 2021
Contact name Valeria Cavalli
E-mail(s) cavalli@pcg.wustl.edu
Organization name Washington University in St. Louis
Department Anatomy and Neurobiology
Street address 660 S. Euclid Ave.
City Saint Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL21572 [miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version]
Samples (11)
GSM2402611 eDRG_Control_Rep1
GSM2402612 eDRG_Control_Rep2
GSM2402613 eDRG_Control_Rep3
This SubSeries is part of SuperSeries:
GSE90477 Gene repression by DNA methylation promotes axon regeneration
Relations
BioProject PRJNA354826

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90475_RAW.tar 8.4 Mb (http)(custom) TAR (of CEL)
GSE90475_normalized_data_with_annotation.txt.gz 152.6 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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