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Series GSE66597 Query DataSets for GSE66597
Status Public on Jan 04, 2016
Title Insights into responses of human astrocytes to H5N1 infection by transcriptional analysis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Increasing evidence demonstrates influenza virus can not only affect the respiratory system, but also infect CNS and lead to CNS disorder and encephalopathy and encephalitis. Astrocytes are the most abundant cells in the CNS, which are capable of producing cytokines and neurotrophic factors and are essential for brain homeostasis and neuronal function. Previous studies suggested that influenza virus can infect astrocytes and induce proinflammatory cytokines response as well as apoptosis. Nevertheless, very few mechanistic data are available regarding host responses to H5N1 infection in astrocytes. In this study, a functional genomics approach was utilized to investigate comprehensive host responses to H5N1 infection in a human astrocyte cell line, U251 cells.
 
Overall design U251 cells were infected by H5N1 at MOI 1 or control . At time points 6, 12, and 24h, total RNA were extracted for microarray experiment. Three replicates were performed at each time point of infection and control infection.
 
Contributor(s) Lin X, Wang R, Sun X, Zhang J, Jin M
Citation(s) 26008703
Submission date Mar 06, 2015
Last update date Jan 23, 2019
Contact name Lin xian
E-mail(s) yoya12@163.com
Organization name Huazhong Agricutural University
Department College of Veterinary Medicine
Lab State Key Laboratory of Agricultural Microbiology
Street address shizishan street,number 1
City wuhan
State/province Hubei
ZIP/Postal code 430070
Country China
 
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (18)
GSM1625995 con6h-rep1
GSM1625996 con6h-rep2
GSM1625997 con6h-rep3
Relations
BioProject PRJNA277411

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66597_RAW.tar 161.4 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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