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Series GSE62301 Query DataSets for GSE62301
Status Public on May 01, 2015
Title Developmental LTbR synergistically activates TLR4 mediated inflammatory RelA/NF-kB response
Organism Mus musculus
Experiment type Expression profiling by array
Summary Developmental signals are known to modulate inflammation. How ever, the mechanistic insight that links developmental and inflammatory signaling remains elusive. In the current study, we identifya critical role of NF-kB system in mediating stimulus specific crosstalk that allows developmental LTbR signals to sustain inflammatory TLR4 induced RelA/NF-kB response and gene expression. LTbR activated non-canonical signaling targets canonical TLR4 induced, nfkb2 encoded p100 not only to deplete inhibitory IkBd/(p100)2, but also to supplement RelA:p52/NF-kB dimers. Robust crosstalk in the gut epithelial cells are important, as crosstalk-defective nfkb2-/- mice succumbed to gut infection by Citrobacter rodentium due to hypo-inflammatory responses. Finally, we present evidence for a crosstalk motif that integrates tissue microenvironment derived developmental cues to ameliorate the pathogen response.
 
Overall design Total RNA from WT early passage MEFs stimulated with ligands LPS, LTbR and LPS+LTbR for 24hrs were analyzed for global gene expression levels
 
Contributor(s) Basak S, Banoth B
Citation(s) 25905673
Submission date Oct 14, 2014
Last update date Jun 14, 2018
Contact name Balaji Banoth
E-mail(s) balajibanoth@nii.ac.in
Organization name National Institute of Immunology
Department Immunology
Lab Systems Immunology Lab
Street address Aruna Asaf Ali Marg
City New Delhi
State/province Delhi
ZIP/Postal code 110067
Country India
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM1525024 WT 0 hr
GSM1525025 WT LPS 24 hrs
GSM1525026 WT LTbR 24 hrs
Relations
BioProject PRJNA263799

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62301_RAW.tar 3.1 Mb (http)(custom) TAR
GSE62301_non-normalized.txt.gz 1.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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