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Series GSE189968 Query DataSets for GSE189968
Status Public on Dec 04, 2021
Title MicroRNAs Mediate Insecticide Tolerance in Spodoptera frugiperda
Organism Spodoptera frugiperda
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Spodoptera frugiperda is the world’s major agricultural pests, and has the distinctive features of high fecundity, strong migratory capacity and high resistance to most insecticides. At present, the control of S. frugiperda in China relies mainly on the spraying of chemical insecticides. MicroRNAs (miRNAs) are a class of small, single-stranded, non-coding RNAs, and play crucial regulatory roles in various physiological processes, including the insecticide resistance in insects. However, little is known about the regulatory roles of miRNAs on the resistance of S. frugiperda to insecticides. In the present research, the miRNAs that were differentially expressed after cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole treatment were analyzed by RNA-Seq. A total of 504 miRNAs were systematically identified from S. frugiperda, and 24, 22, 31 and 30 miRNAs were differentially expressed after treatments of cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole. GO and KEGG enrichment analyses were used to predict the function of differentially expressed miRNAs’ target genes. Importantly, ten miRNAs were significantly differentially expressed among the treatments of three insecticides. MiR-278-5p, miR-13b-3p, miR-10485-5p and miR-10483-5p were significantly down-regulated among the treatments of three insecticides by RT-qPCR. Furthermore, overexpression of miR-278-5p, miR-13b-3p, miR-10485-5p and miR-10483-5p significantly increased the mortality of S. frugiperda to cyantraniliprole and emamectin benzoate. The mortality was significantly increased with spinetoram treatment after overexpression of miR-13b-3p, miR-10485-5p and miR-10483-5p. These results suggest that miRNAs, which are differentially expressed in response to insecticides, may play a key regulatory role in the insecticide resistance in S. frugiperda.
 
Overall design The miRNAs that were differentially expressed after cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole treatment were analyzed in Spodoptera frugiperda.
 
Contributor(s) Zhang J, Yang Y, Zhang Y
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Submission date Dec 02, 2021
Last update date Dec 04, 2021
Contact name jianhua zhang
E-mail(s) zhangjianhua198904@126.com
Organization name Jiangsu Academy of Agricultural Sciences
Street address zhongling street No. 50
City Nanjing
ZIP/Postal code 210014
Country China
 
Platforms (1)
GPL25030 Illumina HiSeq 2500 (Spodoptera frugiperda)
Samples (15)
GSM5710656 ck1
GSM5710657 ck2
GSM5710658 ck3
Relations
BioProject PRJNA785443

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GSE189968_Processed_data.xlsx 85.9 Kb (ftp)(http) XLSX
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Processed data are available on Series record

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