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Series GSE189038 Query DataSets for GSE189038
Status Public on Nov 24, 2021
Title Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae [I]
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary The formation of heterochromatin at HML, HMR, and telomeres in Saccharomyces cerevisiae involves two main steps: Recruitment of Sir proteins to silencers and their spread throughout the silenced domain. For the following datasets, we created a fusion protein between the heterochromatin protein Sir3 and the non-site-specific bacterial adenine methyltransferase M.EcoGII, with or without a 3xV5 epitope at the C-terminus. We performed ChIP-seq experiments (immunoprecipitated Sir3-M.EcoGII-3xV5) and MeDIP-seq experiments (immunoprecipitated m6A methylated DNA). We also used a temperature-sensitive allele of SIR3 (sir3-8) fused to M.ECOGII to induce m6A methylation for MeDIP-seq.
 
Overall design 24 samples total. Input and IP data for each experiment are included.
 
Contributor(s) Brothers M, Rine J
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Submission date Nov 17, 2021
Last update date Dec 03, 2021
Contact name Molly Brothers
E-mail(s) molly_brothers@berkeley.edu
Organization name University of California, Berkeley
Department Molecular and Cell Biology
Lab Rine Lab (440 Barker Hall)
Street address UC Berkeley 440 Barker Hall
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platforms (1)
GPL22715 Illumina MiniSeq (Saccharomyces cerevisiae)
Samples (24)
GSM5693567 210122_Crabby_Sir3-MEcoGII-3xV5_input
GSM5693568 210122_Crabby_Sir3-MEcoGII-3xV5_ChIP
GSM5693569 200808_Chase_no-EcoGII_m6A_input
This SubSeries is part of SuperSeries:
GSE190137 Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae
Relations
BioProject PRJNA781240
SRA SRP346595

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Supplementary file Size Download File type/resource
GSE189038_RAW.tar 1.5 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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