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Series GSE184564 Query DataSets for GSE184564
Status Public on Dec 03, 2021
Title Spatial transcriptomic studies of the small intestine from WT and DDX5△IEC (KO) mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary DDX5, a DEAD box containing RNA binding protein, is involved in multiple aspects of RNA metabolism and is abundantly expressed in all subsets of the intestinal epithelial cells (IEC). Here, we characterized the DDX5-dependent transcriptome in the small intestine. In the DDX5△IEC tissue, we found that secretory IEC lineage progenitor containing spots (Atoh1hi and Sox4hi) had reduced expression of Pou2f3, a master regulator of a subset of IECs called tuft cells. As a result of the loss of Pou2f3 expression in the progenitors, DDX5△IEC tissue harbored lower number of tuft cell containing spots (Dclk1hi) compare to WT tissues.
 
Overall design One intestine tissue from each genotype were obtained from 8wk old cohoused mice. Results of ileum epithelium from DDX5△IEC mutant mice were compared to those from WT mice.
 
Contributor(s) Long T, Lemolo A, Telese F, Huang WM
Citation(s) 34853057
Submission date Sep 21, 2021
Last update date Dec 03, 2021
Contact name Wendy Huang
E-mail(s) wjh003@ucsd.edu
Organization name University of California San Diego
Department Cellular and Molecular Medicine
Street address 9500 Gilman Drive #0651
City San Diego
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM5592663 KO_1883
GSM5592664 WT_1884
Relations
BioProject PRJNA765044
SRA SRP338113

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184564_KO_metrics_summary.csv.gz 525 b (ftp)(http) CSV
GSE184564_WT_metrics_summary.csv.gz 529 b (ftp)(http) CSV
GSE184564_WTonly_Lgr5+_Atoh1+_Sox4+_Dclk1+.csv.gz 1.8 Kb (ftp)(http) CSV
GSE184564_WTvsKO_Atoh1+.csv.gz 1.6 Kb (ftp)(http) CSV
GSE184564_WTvsKO_Chga.csv.gz 4.6 Kb (ftp)(http) CSV
GSE184564_WTvsKO_DCLK1+.csv.gz 784 b (ftp)(http) CSV
GSE184564_WTvsKO_Klf4+.csv.gz 3.4 Kb (ftp)(http) CSV
GSE184564_WTvsKO_Lyz1+.csv.gz 3.1 Kb (ftp)(http) CSV
GSE184564_WTvsKO_Sox4+.csv.gz 1.0 Kb (ftp)(http) CSV
GSE184564_barcodes.tsv.gz 55.2 Kb (ftp)(http) TSV
GSE184564_features.tsv.gz 284.1 Kb (ftp)(http) TSV
GSE184564_matrix.mtx.gz 30.2 Mb (ftp)(http) MTX
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Processed data are available on Series record

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