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Series GSE179702 Query DataSets for GSE179702
Status Public on Nov 23, 2021
Title Chemically-induced epimutagenesis allows bypassing reproductive barriers in hybrid seeds [BiSulfite-seq]
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Summary The "triploid block" prevents interploidy hybridizations in flowering plants, and is characterized by failure in endosperm development and function, arrest in embryogenesis, and seed collapse. Many genetic components of triploid seed lethality have been successfully identified in the model plant Arabidopsis thaliana, most notably the paternally expressed imprinted genes (PEGs) that are up-regulated in the tetraploid endosperm with paternal excess. Previous studies have shown that the paternal epigenome is a key determinant of the triploid block response, as the loss of DNA methylation in diploid pollen suppresses the triploid block almost completely. Here, we demonstrate that triploid seed abortion is bypassed in plants treated with the DNA methyltransferase inhibitor 5-Azacytidine during seed germination and early plant growth. We have identified strong suppressor lines showing transgenerational inheritance of hypomethylation in CG context, as well as normalized expression of PEGs. Importantly, differentially methylated loci segregate in the progeny of "epimutagenized" plants, which may allow the identification of epialleles involved in the triploid block response in future studies. Finally, we demonstrate that chemically-induced epimutagenesis allows bypassing interploidy hybridizations in Arabidopsis and interspecific hybridization barriers in crosses between Capsella species, thus potentially emerging as a novel strategy for seed-based breeding of triploids and interspecific hybrids with agronomical interest.
 
Overall design Whole-genome bisulfite sequencing of Arabidopsis seedlings treated with 5-Azacytidine
 
Contributor(s) Borges F
Citation(s) 34792584
Submission date Jul 07, 2021
Last update date Nov 25, 2021
Contact name Filipe Borges
E-mail(s) filipe.borges@inrae.fr
Organization name Institut Jean-Pierre Bourgin - INRAE
Street address Route de St-Cyr (RD10)
City Versailles
ZIP/Postal code 78026
Country France
 
Platforms (1)
GPL28290 DNBSEQ-G400 (Arabidopsis thaliana)
Samples (14)
GSM5429627 untreated jas-3#1
GSM5429628 untreated jas-3#2
GSM5429629 DMSO#1
This SubSeries is part of SuperSeries:
GSE179704 Chemically-induced epimutagenesis allows bypassing reproductive barriers in hybrid seeds
Relations
BioProject PRJNA744574
SRA SRP327419

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE179702_RAW.tar 553.9 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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