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Series GSE179491 Query DataSets for GSE179491
Status Public on Sep 15, 2021
Title Brd4 regulates the homeostasis of CD8+ T-lymphocytes and their proliferation in response to antigen stimulation [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goals of this study are to compare the differentially expressed genes between in wild type and Brd4-/- CD8+T cells.
Methods: RNA extracted from wild type and Brd4-/- CD8+T cells was used to generate RNA-seq library,followed by sequencing.
Results: In naïve CD8+T cells, Brd4 deletion casued 956 downregulated genes and 841 upregulated genes. In in vitro-activated CD8+T cells(18hr),Brd4 deletion resulted in 1182 downregulated genes and 789 upregulated genes. Importantly,we noticed that Brd4 is required for the migration- and glucose-related genes. expression
Conclusions: Our study represents the first detailed analysis of transcriptomes in wild type and Brd4-/- CD8+T cells. Our results show that Brd4 deletion resulted in more downregulated genes exceeding the upregulated genes and the downregulation of many migration- and glucose-ralated genes. We conclude that Brd4-mediated gene expresssion program promotes glucose metabolism and cell migartion.
 
Overall design mRNA profiles of wild type (WT) and Brd4-/- CD8+T cells
 
Contributor(s) Peng Z, Zhang H
Citation(s) 34512660
Submission date Jul 06, 2021
Last update date Sep 15, 2021
Contact name Peng zhi lin
E-mail(s) jlulin@126.com
Phone 15626213510
Organization name Sun-Yat sen university
Department zhongshan school of medicine
Lab Zhang
Street address 74 Zhongshan 2 Rd
City 广州
ZIP/Postal code 510080
Country China
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM5419301 Naïve WT CD8+T cells rep1
GSM5419302 Naïve KO CD8+T cells rep1
GSM5419303 18hr WT CD8+T cells rep1
This SubSeries is part of SuperSeries:
GSE179492 Brd4 regulates the homeostasis of CD8+ T-lymphocytes and their proliferation in response to antigen stimulation.
Relations
BioProject PRJNA744057
SRA SRP327094

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE179491_gene_expression.txt.gz 1.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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