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Series GSE171975 Query DataSets for GSE171975
Status Public on Nov 28, 2021
Title Ultradian Molecular Rhythms Emerge in the Absence of a Circadian Clock
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In the chronobiology field, a fundamental dichotomy exists to explain daily rhythmicity of biological processes: these can be elicited in response to cyclic extrinsic/environmental signals such as light, or driven endogenously by the circadian clock. In mammals, the circadian clock ticks in almost every cell of the body, and functions based on a network of transcription-translation feedback loops. The PI3K-AKT signaling pathway relays environmental information of nutritional/metabolic state to regulate cell size and proliferation. AKT, a Serine/Threonine protein kinase, is activated by phosphorylation, where phospho-serine 473 (pAKT) serves as a hallmark for its activation. Following activation, it proceeds to phosphorylate dozens of target proteins that convey the signal to regulate gene expression and other key cellular functions. Overall, this pathway is widely known to be activated in response to feeding related signals, and previous studies in mice found elevated pAKT levels in correspondence with food ingestion. However, it is still unknown whether this can (also) be driven through intrinsic mechanisms, such as the circadian clock. Here, we inspected daily activation of AKT both in cultured cells and animal models. Unexpectedly, we found, that neither environmental cues nor the circadian clock were necessary for pAKT rhythms, which exhibited ultradian, rather than circadian, cycles of phosphorylation. In addition, hepatic gene expression also exhibited short rhythms in clock disrupted mice, corresponding with AKT related genes/functions. Reciprocally, inhibition of AKT phosphorylation did not affect the rhythmicity of the circadian clock. Overall, our findings uncover temporal regulation of AKT activation and reveal ultradian molecular rhythmicity that cycles independently of the canonical circadian clock.
Overall design RNA-sequencing on either wild type (WT) or Per1/2-/- (PerDKO) mice, kept in constant Darkness. RNA was extracted from livers collected over a two-day time course, in 4h intervals.
Contributor(s) Aviram R, Manella G, Asher G, Golik M
Citation(s) 34968386
Submission date Apr 13, 2021
Last update date Jan 10, 2022
Contact name Gal Manella
Organization name Weizmann Institute of Science
Street address 234 Herzel St.
City Rehovot
State/province Israel
ZIP/Postal code 7610001
Country Israel
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (140)
GSM5239166 Liver_WT_DD_CT0_A
GSM5239167 Liver_WT_DD_CT0_B
GSM5239168 Liver_WT_DD_CT0_C
BioProject PRJNA721602
SRA SRP314631

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Supplementary file Size Download File type/resource
GSE171975_Liver_BMALKO_reads.xlsx 2.6 Mb (ftp)(http) XLSX
GSE171975_R370_Liver_WT_reads.xlsx 10.1 Mb (ftp)(http) XLSX
GSE171975_R389_Liver_PerDKO_reads.xlsx 10.1 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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