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Series GSE156174 Query DataSets for GSE156174
Status Public on Dec 01, 2021
Title RNA-sequencing of skeletal stem cells (SSC) and mesenchymal stem cells (MSC) from hindlimb unloaded and reloaded mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Disuse osteoporosis (DO) occurs in response to mechanical unloading of the skeleton and results in structural changes that compromise bone strength leading to increased fracture risk. Although skeletal stem cells (SSCs) are the main source of osteoblasts and adipocytes in adult bone marrow, whether DO impacts SSC function has not been established. Here, three-month-old male C57BL/6 mice were subjected to a hindlimb unloading (HU) model of disuse osteoporosis for 8 or 14 weeks by tail suspension. Control mice (ambulatory or AMB) were allowed normal use of their hindlimbs. An additional cohort underwent an 8-week recovery period after 8-weeks of unloading. Bone marrow-derived, LepR+ skeletal stem cells (SSCs) were isolated by FACS (n=3 or 4) and pooled prior to RNA isolation. Where indicated, cultured, immuno-depleted, bone marrow-derived mesenchymal stem cells (MSCs) were also collected (n=3) and pooled prior to RNA isolation. Generated libraries were subjected to NGS RNA-seq analysis on the Illumina NextSeq 500. These data delineate unique physiological adaptations to prolonged unloading, demonstrate that DO results in increased leptin expression and sympathetic signaling within the BM niche.
Overall design RNA-seq analysis of RNA derived from hindlimb unloaded and reloaded mouse FACS-isolated for LepR+ SSCs or cultured and immuno-depleted MSCs.
Contributor(s) Phinney DG, Booker CN, Haga CL
Citation(s) 34836964
Submission date Aug 13, 2020
Last update date Dec 02, 2021
Contact name Donald G. Phinney
Organization name The Scripps Research Institute
Department Molecular Medicine
Street address 130 Scripps Way Bldg A215
City Jupiter
State/province Florida
ZIP/Postal code 33453
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM4726006 8wk ambulatory MSC
GSM4726007 8wk ambulatory SSC
GSM4726008 8wk hindlimb unloaded MSC
BioProject PRJNA656995
SRA SRP277358

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Supplementary file Size Download File type/resource
GSE156174_RAW.tar 3.0 Mb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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