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Series GSE152371 Query DataSets for GSE152371
Status Public on Dec 01, 2021
Title Small non-coding RNA sequencing of human monocyte-derived macrophages
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The goal of this experiment was to identify the differential expression of microRNAs in human polarised macrophages. We used monocyte-derived macrophages isolated from 8 different healthy invididuals. Differentiated macrophages were polarised towards the pro-inflammatory M1-like phenotype, by using LPS and INF-𝛾. Total RNA was extracted and sent for small-RNA sequencing to the company Novogene.
 
Overall design Identification of microRNAs differentially expressed between unpolarised and M1 polarised macrophages
 
Contributor(s) Niespolo C, Deshmukh SR, Sudbery IM, Wilson HL, Kiss-Toth E
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Submission date Jun 12, 2020
Last update date Dec 01, 2021
Contact name Chiara Niespolo
E-mail(s) c.niespolo@sheffield.ac.uk, chiara.niespolo@gmail.com
Organization name University of Sheffield
Department Infection, Immunity and Cardiovascular Disease
Lab Endre Kiss-Toth Group
Street address Beech Hill Road
City Sheffiled
ZIP/Postal code S10 2RX
Country United Kingdom
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (16)
GSM4613607 Sample 1 M0
GSM4613608 Sample 1 M1
GSM4613609 Sample 2 M0
Relations
BioProject PRJNA639121
SRA SRP267092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152371_raw_counts_miRNAseq.txt.gz 1.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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