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Series GSE141393 Query DataSets for GSE141393
Status Public on Dec 01, 2021
Title MicroRNA-mRNA expression profiles and functional networks of Submandibular Gland in type 2 diabetic db/db mice [seq]
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Abstract: Background: Hyposalivation is one of the common symptoms of diabetes. Although the micro ribonucleic acid (miRNA) has recently been identified to play a role in the pathogenesis of diabetes, the specific effects of miRNAs on diabetic salivary glands remain unclear. This work was aimed to investigate the expression profiles of miRNAs and identify miRNA‐mRNA network in submandibular gland (SMG) in db/db mice. Methods: miRNA and mRNA expressional profiles were analyzed by microarray technology from the SMG of db/db mice and db/m mice. Significant fold changes in miRNAs and mRNAs were confirmed by real-time quantitative reverse transcription-polymerase chain reaction. Bioinformatic analyses were performed to identify the critical biological functions and signaling pathways involved in diabetes-mediated hyposalivation and to identify the functional networks formed between differentially expressed (DE) miRNAs and mRNAs. Results: A total of 28 DE miRNAs and 1146 DE mRNAs were identified of the SMG in db/db mice and db/m mice. Gene ontology (GO) analysis and KEGG pathway analysis demonstrated that DE miRNAs were highly associated with terms related to diverse biological processes and signaling pathways. Of the related pathways, TGF-beta signaling pathway and tight junction pathway were notable. The constructed network indicated interactions between 11 miRNAs and 42 mRNAs. Autophagy pathway is worth studying, especially the dynamic network of miRNA and mRNA. In addition, AKT3 and PIK3CD may play important roles in the development of diabetes-mediated hyposalivation. Conclusions: Our research describes the miRNA and mRNA expression profiles and functional networks involved in SMGs from db/db mice. These results provide possible molecular mechanisms and may help to elucidate possible molecular therapeutic targets for the treatment of diabetes-induced hyposalivation. Key words: diabetes, submandibular gland, miRNA, mRNA, miRNA–mRNA network, microarray
Overall design Detection of expression profiles of miRNA and mRNA in submandibular glands of db/m and db/db mice, and analysis of GO and KEGG pathways.
Contributor(s) Xiang R, Liu H, Shi X
Citation(s) 34831451
Submission date Dec 03, 2019
Last update date Dec 01, 2021
Contact name huimin liu
Phone 13146619655
Organization name Peking University
Department Peking University Health Science Center
Lab Department of Physiology and Pathophysiology
Street address 38 Xueyuan Rd , Haidian District
City Beijing
State/province Beijing
ZIP/Postal code 100191
Country China
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM4202311 db-m-1
GSM4202312 db-m-2
GSM4202313 db-m-3
This SubSeries is part of SuperSeries:
GSE141412 MicroRNA-mRNA expression profiles and functional networks of Submandibular Gland in type 2 diabetic db/db mice
BioProject PRJNA593262
SRA SRP234573

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Supplementary file Size Download File type/resource
GSE141393_miRNA_Expression.xls.gz 79.4 Kb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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