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Series GSE138815 Query DataSets for GSE138815
Status Public on Oct 20, 2021
Title Histone demethylase KDM6A promotes somatic cell reprogramming by epigenetically regulate PTEN and IL-6 signal pathway [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Aberrant epigenetic reprogramming is one of the major barriers for somatic cell reprogramming. Although our previous study has demonstrated that KDM6A, a H3K27me3 demethylase, could improve nuclear reprogramming efficiency, but its role in somatic cell reprogramming and the mechanism remain unclear. In this study, we demonstrated that overexpression of Kdm6a could improve iPSC reprogramming efficiency in a demethylase enzymatic activity-dependent manner. And Kdm6a overexpression changed the gene expression profile and metabolic pattern through erasing the repressive H3K27me3 in fibroblast. Besides, we proved that KDM6A could promote IL-6 expression in MEF cells and the secreted IL-6 also could improve iPSC reprogramming efficiency. Furthermore, we found that KDM6A could promote PTEN expression, further regulate p-AKT and p-mTOR levels, which favor the reprogramming, and interference of PTEN could counteracted the promotion effect of KDM6A. Taken together, our data revealed that KDM6A could promote iPSC reprogramming efficiency by accelerate the change of gene expression profile and metabolic pattern in a demethylation-activity-dependent manner, which may give insight to the relationship between epigenetic modification and somatic cell reprogramming.
 
Overall design 48 hrs after infection or 72h post DOX was added, the total RNA of 4F2A MEFs was harvested by using TRIzol reagent (Invitrogen), each group was prepared with three parallel replicates. Then, all the samples were sent to BGI Corporation (China) for further RNA-seq analysis via BGISEQ-500 sequencer. The GO and KEGG pathway analyses were performed based on the KEGG database on the Dr. Tom network platform of BGI (http://report.bgi.com).
 
Contributor(s) Jiang Q, Lei L
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Submission date Oct 14, 2019
Last update date Oct 20, 2021
Contact name Qi Jiang
E-mail(s) jiangqi1002@126.com
Organization name Harbin Medical University
Department Department of histology and embryology
Street address baojian road 157
City harbin
State/province heilongjiang
ZIP/Postal code 150081
Country China
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (12)
GSM4119859 day 0 con rep1
GSM4119860 day 0 con rep2
GSM4119861 day 0 con rep3
This SubSeries is part of SuperSeries:
GSE138817 Histone demethylase KDM6A promotes somatic cell reprogramming by epigenetically regulate PTEN and IL-6 signal pathway
Relations
BioProject PRJNA577418
SRA SRP225522

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138815_gene_expression.txt.gz 648.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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