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Series GSE129247 Query DataSets for GSE129247
Status Public on Dec 01, 2021
Title Down-regulation of atherosclerosis signaling in patients treated with on-line haemodiafiltration may contribute to lower cardiovascular risk in hemodialysis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Recent studies suggest that on-line hemodiafiltration (OL-HDF) may reduce the progression of dialysis-related cardiovascular diseases, but the molecular mechanisms underlying this effect are unclear. The aim of our study was to identify, through a high-throughput approach, differences in gene expression profiles in peripheral blood mononuclear cells (PBMCs) associated with OL-HDF and bicarbonate dialysis (BHD). The transcriptomic profile was investigated in PBMCs isolated from 8 patients on OL-HDF and 8 patients on BHD by microarray analysis. The results were evaluated by statistical and functional pathway analysis and validated by qPCR in an independent cohort of patients (OL-HDF N=20, BHD n=20, testing group). Eight-hundred and sixty-eight genes were differentially expressed in the comparison between OL-HDF and BHD. Thirty-seven functional gene networks were identified and atherosclerosis signaling was the top canonical pathway regulated by OL-HDF. Among the genes modulated by OL-HDF in this pathway there were PDGF A chain (FC=-2.13), Clusterin (FC=-2.14), Monoamine Oxidase A (MAO-A, FC=-2.43), IL-6 (FC=-1.56), VEGF C (FC=-1.83) and Apolipoprotein E (Apo-E) (FC=+1.69). qPCR, performed in the testing-group, confirmed the microarray data. In the same set of patients PDGF AA, IL-6 and VEGF C serum levels were significantly lower in the OL-HDF group. Finally, 10 BHD patients were switched to OL-HDF and PBMC were harvested after 6 months. The qPCR results from this perspective group further confirmed the modulation of atherosclerotic genes observed in the cross-sectional analysis. In conclusion, OL-HDF might contribute to cardiovascular risk reduction through the modulation of specific proteins involved in atherosclerotic disease
 
Overall design We performed microarray experiments in PBMCs isolated from 8 patients on OL-HDF and 8 patients on BHD. BHD subjects were treated three times per week with 240-min ultrapure bicarbonate based dialysate, using a synthetic high-flux membrane. OL-HDF was performed using a blood flow rate of >300 mL/min and a dialysate flow rate of 500 mL/min and synthetic high-flux dialysers, plus the on-line production of ultrapure bicarbonate-buffered substitution volume in post-dilution mode with a target convective volume of ≥22 L. Eight patients/treatment group were randomly selected and assigned to the microarray analysis group.
 
Contributor(s) Simone S, Chieti A, Rascio F, Pontrelli P, Castellano G, Corciulo R, Stallone G, Infante B, Gesualdo L, Grandaliano G, Pertosa G
Citation(s) 33761122
Submission date Apr 02, 2019
Last update date Dec 02, 2021
Contact name Paola Pontrelli
E-mail(s) paolapontre@libero.it
Phone +390805478868
Organization name University of Bari
Department Department of Emergency and Organ Transplantation
Lab Nephrology Unit
Street address Piazza G.Cesare 11
City Bari
ZIP/Postal code 70124
Country Italy
 
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (16)
GSM3703518 PBMCs_OL-HDF_patient 1
GSM3703519 PBMCs_OL-HDF_patient 2
GSM3703520 PBMCs_OL-HDF_patient 3
Relations
BioProject PRJNA530595

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129247_RAW.tar 47.7 Mb (http)(custom) TAR (of TXT)
GSE129247_normalized_data_with_gene_name.txt.gz 4.9 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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