NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE124940 Query DataSets for GSE124940
Status Public on Jan 11, 2019
Title The Impact of Wheat Rht-B1b Semi-Dwarfing Allele on Photosynthesis and Seed Development under Field Conditions
Organism Triticum aestivum
Experiment type Expression profiling by high throughput sequencing
Summary The Reduced Height (Rht) genes formed the basis for the green revolution in wheat by decreasing plant height and increasing productive tillers. There are two current widely used Rht mutant alleles, Rht-B1b and Rht-D1b. Both reduce plant height by 20% and increase seed yield by 5-10%. They are also associated with decreased seed size and protein content. Here we tested the degree to which Rht-B1b impacts flag leaf photosynthetic rates and carbon and nitrogen partitioning to the flag leaf and grain during grain fill under field conditions using near isogenic lines (NILs) that were either standard height (Rht-B1a) or semi-dwarf (Rht-B1b). The results demonstrate that at anthesis, Rht-B1b reduces flag leaf photosynthetic rate per unit area by 18% and chlorophyll A content by 23%. Rht-B1b significantly reduced grain protein beginning at 14 days post anthesis with the greatest difference seen at 21 days post anthesis (DPA) (12%). Rht-B1b also significantly decreased individual seed weight beginning at 21 DPA and by 15.2% at 28 DPA. Global expression analysis using RNA extracted from developing leaves and stems demonstrated that genes associated with carbon and nitrogen metabolism are not substantially altered by Rht-B1b. From this study, we conclude that Rht-B1b reduces flag leaf photosynthetic rate at flowering while changes in grain composition begin shortly after anthesis.
 
Overall design Total RNA was collected from leaf and stem tissue from plants at 14 days past anthesis. Three samples were taken from plants carrying the semi-dwarfing allele Rht-B1b and three samples were taken from plants without the semi-dwarfing allele.
 
Contributor(s) Jobson EM, Johnston RE, Oiestad AJ, Martin JM, Giroux MJ
Citation(s) 30778362
Submission date Jan 10, 2019
Last update date Feb 25, 2019
Contact name Michael Giroux
E-mail(s) mgiroux@montana.edu
Organization name Montana State University
Department Plant Sciences
Street address 119 Plant Bioscience Building
City Bozeman
State/province Montana
ZIP/Postal code 59717
Country USA
 
Platforms (1)
GPL18862 Illumina HiSeq 2500 (Triticum aestivum)
Samples (24)
GSM3559740 Leaf_Fortuna10_rep1
GSM3559741 Leaf_Fortuna10_rep2
GSM3559742 Leaf_B1b11_rep1
Relations
BioProject PRJNA514367
SRA SRP178272

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124940_9.30.2016_Select_Genes_Default.astar.gz 51.8 Kb (ftp)(http) ASTAR
GSE124940_RNA_SEQ_edited_7.19.xlsx 498.8 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap