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Series GSE121747 Query DataSets for GSE121747
Status Public on Oct 20, 2021
Title Identification of a T cell lymphoma in plkcGAPDH transgénic mice (RNA-Seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) is emerging as a key player in T-cell development, function and cancer. Here we investigated the role of GAPDH in T-cell development/function by overexpressing GAPDH in the T-cell lineage. Aged mice developed: 1) splenomegaly, 2) enlarged lymph nodes, 3) lymphocyte-infiltrations in the liver and bone marrow. All showed an increase of strongly proliferating and clonal Tfh CXCR5+PD1highCD4+-T cells associated with germinal center B cells and inflammatory cytokine-release. Gene-set-expression-analysis confirmed that this lymphoma was equivalent to human angioimmunoblastic T-cell lymphoma (AITL). Mechanistically, GAPDH induced NF-B pathway in the murine AITL in vivo inhibition of NF-B combined with anti-PD1 increased mice survival and cancer immune response. GAPDH-dependent modulation of NF-B in T-cells allowed to model AITL-disease and evaluate treatments.
 
Overall design mRNA profiles of lymphomas from plckGAPDH mice were generated by RNA sequencing (RNASeq), in triplicate, using Illumina GAIIx.
 
Contributor(s) Paquet A, Pons N, Lebrigand K, Barbry P, Tosolini M, Verhoeyen E
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Submission date Oct 24, 2018
Last update date Oct 20, 2021
Contact name Kevin Lebrigand
Organization name IPMC/CNRS
Lab Functional Genomics Platform of Nice-Sophia-Antipolis, France.
Street address 660 route des lucioles
City Valbonne - Sophia-Antipolis
ZIP/Postal code 06560
Country France
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (9)
GSM3445022 WT5
GSM3445023 WT4
GSM3445024 WT1
This SubSeries is part of SuperSeries:
GSE121748 Identification of a T cell lymphoma in plkcGAPDH transgenic mice
Relations
BioProject PRJNA498574
SRA SRP166994

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Supplementary file Size Download File type/resource
GSE121747_RNAseq_Verhoeyen.txt.gz 755.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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