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Series GSE121592 Query DataSets for GSE121592
Status Public on Oct 20, 2021
Title Identification of a T-cell lymphoma in plkcGAPDH transgenic mice (Exome)
Organism Mus musculus
Experiment type Genome variation profiling by high throughput sequencing
Summary Glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) is emerging as a key player in T-cell development, function and cancer. Here we investigated the role of GAPDH in T-cell development/function by overexpressing GAPDH in the T-cell lineage. Aged mice developed: 1) splenomegaly, 2) enlarged lymph nodes, 3) lymphocyte-infiltrations in the liver and bone marrow. All showed an increase of strongly proliferating and clonal Tfh CXCR5+PD1highCD4+-T cells associated with germinal center B cells and inflammatory cytokine-release. Gene-set-expression-analysis confirmed that this lymphoma was equivalent to human angioimmunoblastic T-cell lymphoma (AITL). Mechanistically, GAPDH induced NF-kB pathway in the murine AITL in vivo inhibition of NF-kB combined with anti-PD1 increased mice survival and cancer immune response. GAPDH-dependent modulation of NF-kB in T-cells allowed to model AITL-disease and evaluate treatments.
 
Overall design Whole exome sequencing of CD4+ T-cells isolated from plkcGAPDH transgenic mice
 
Contributor(s) Verhoeyen E, Mhaidly R, Shuran CY
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Submission date Oct 22, 2018
Last update date Oct 21, 2021
Contact name Kevin Lebrigand
Organization name IPMC/CNRS
Lab Functional Genomics Platform of Nice-Sophia-Antipolis, France.
Street address 660 route des lucioles
City Valbonne - Sophia-Antipolis
ZIP/Postal code 06560
Country France
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM3439844 A249
GSM3439845 A331
GSM3439846 A508
This SubSeries is part of SuperSeries:
GSE121748 Identification of a T cell lymphoma in plkcGAPDH transgenic mice
Relations
BioProject PRJNA498681
SRA SRP166976

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE121592_RAW.tar 3.6 Mb (http)(custom) TAR (of VCF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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