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Series GSE121592 Query DataSets for GSE121592
Status Public on Oct 20, 2021
Title Identification of a T-cell lymphoma in plkcGAPDH transgenic mice (Exome)
Organism Mus musculus
Experiment type Genome variation profiling by high throughput sequencing
Summary Glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) is emerging as a key player in T-cell development, function and cancer. Here we investigated the role of GAPDH in T-cell development/function by overexpressing GAPDH in the T-cell lineage. Aged mice developed: 1) splenomegaly, 2) enlarged lymph nodes, 3) lymphocyte-infiltrations in the liver and bone marrow. All showed an increase of strongly proliferating and clonal Tfh CXCR5+PD1highCD4+-T cells associated with germinal center B cells and inflammatory cytokine-release. Gene-set-expression-analysis confirmed that this lymphoma was equivalent to human angioimmunoblastic T-cell lymphoma (AITL). Mechanistically, GAPDH induced NF-kB pathway in the murine AITL in vivo inhibition of NF-kB combined with anti-PD1 increased mice survival and cancer immune response. GAPDH-dependent modulation of NF-kB in T-cells allowed to model AITL-disease and evaluate treatments.
Overall design Whole exome sequencing of CD4+ T-cells isolated from plkcGAPDH transgenic mice
Contributor(s) Verhoeyen E, Mhaidly R, Shuran CY
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Submission date Oct 22, 2018
Last update date Oct 21, 2021
Contact name Kevin Lebrigand
Organization name IPMC/CNRS
Lab Functional Genomics Platform of Nice-Sophia-Antipolis, France.
Street address 660 route des lucioles
City Valbonne - Sophia-Antipolis
ZIP/Postal code 06560
Country France
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM3439844 A249
GSM3439845 A331
GSM3439846 A508
This SubSeries is part of SuperSeries:
GSE121748 Identification of a T cell lymphoma in plkcGAPDH transgenic mice
BioProject PRJNA498681
SRA SRP166976

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE121592_RAW.tar 3.6 Mb (http)(custom) TAR (of VCF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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