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Series GSE113614 Query DataSets for GSE113614
Status Public on Dec 01, 2021
Title Characterization of a newly-identified myelinating oligodendroglial population expressing NDRG1 in the adult mouse central nervous system 
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Oligodendrocytes are the main myelinating and remyelinating cells of the central nervous system. We identified N-myc downstream regulated gene family member 1 (NDRG1) as a specific mature marker of the oligodendroglial lineage, regulated by mTORC2 kinase activity. By generating a transgenic reporter murine line, we managed to precisely characterize the effect of cuprizone-induced treatment on myelinating cells. We also created a new and adult-specific whole genome transcriptomic profile of the oligodendroglial lineage, from proliferative OPC (using P60 PDGFRa::GFP sorted cells), to pre-myelinating OL (using P60 PLP::GFP sorted cells) and then mature OL (using P60 NDRG1-eGFP sorted cells). This new reporter murine line and its associated RNA-Sequencing dataset could help us better understand the specific biology of adult myelinating oligodendrocytes, compared to previously published developmental tools and databases.
 
Overall design mRNA profiles of FAC-sorted P60 PDGFRa::GFP, P60 PLP::GFP and P60 NDRG1-eGFP purified cell samples from mouse brains were generated by RNA-sequencing, in triplicate, using Illumina HiSeq 2500.
 
Contributor(s) Moyon S, Castro K, Patzig J, Gacias M, Magri L, Casaccia P
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Submission date Apr 24, 2018
Last update date Dec 02, 2021
Contact name Sarah Moyon
E-mail(s) smoyon@gc.cuny.edu
Organization name CUNY ASRC
Department Neuroscience
Lab Casaccia
Street address 85 St Nicholas Terrace
City New York
State/province NY
ZIP/Postal code 10031
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
GSM3110139 P60 brain oligodendrocyte progenitor cells_RNASeq_n1
GSM3110140 P60 brain oligodendrocyte progenitor cells_RNASeq_n2
GSM3110141 P60 brain oligodendrocyte progenitor cells_RNASeq_n3
Relations
BioProject PRJNA453399
SRA SRP142537

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113614_RAW.tar 790.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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