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Soares adult brain N2b5HB55Y

Identifiers
BioSample: SAMN00154914; EST: LIBEST_000300
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
sexMale
development stage55-year old
lab hostDH10B (ampicillin resistant)
tissuebrain
vectorpT7T3D (Pharmacia) with a modified polylinker
v_typephagemid
re_1Not I
re_2Eco RI
Description

1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 53. Library constructed by Bento Soares and M.Fatima Bonaldo. The adult brain RNA was provided by Dr. Donald H. Gilden. Tissue was acquired 17-18 hours after death which occurred in consequence of a ruptured aortic aneurysm. RNA was prepared from a pool of tissues representing the following areas of the brain: frontal, parietal, temporal and occipital cortex from the left and right hemispheres, subcortical white matter, basal ganglia, thalamus, cerebellum, midbrain, pons and medulla.

Submission
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Inge Arlart; 1995-05-18
Accession:
SAMN00154914
ID:
154914

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