[Spatial structure of a Fab-fragment of a monoclonal antibody to human interleukin-2 in two crystalline forms at a resolution of 2.2 and 2.9 angstroms]

A V Fokin; P V Afonin; I Iu Mikhaĭlova; I N Tsygannik; T Iu Mareeva; V A Nesmeianov; W Pangborn; N Lee; W Duax; E Siszak; V Z Pletnev

[Spatial structure of a Fab-fragment of a monoclonal antibody to human interleukin-2 in two crystalline forms at a resolution of 2.2 and 2.9 angstroms]

Bioorg Khim. 2000 Aug;26(8):571-8.

[Article in Russian]

Authors

A V Fokin¹, P V Afonin, I Iu Mikhaĭlova, I N Tsygannik, T Iu Mareeva, V A Nesmeianov, W Pangborn, N Lee, W Duax, E Siszak, V Z Pletnev

Affiliation

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

PMID: 11040993

Abstract

The three-dimensional structure of the antigen-binding fragment of a monoclonal antibody to human interleukin-2 was determined in two crystal forms by the X-ray method of molecular replacement at 2.2 and 2.9 A resolutions. The spatial structure of the protein and the stereochemistry of its antigen-binding site were analyzed.

Publication types

English Abstract

MeSH terms

Amino Acid Sequence
Antibodies, Monoclonal / chemistry*
Antibodies, Monoclonal / immunology
Antigens / immunology
Binding Sites, Antibody
Crystallography, X-Ray
Humans
Immunoglobulin Fab Fragments / chemistry*
Immunoglobulin Fab Fragments / immunology
Interleukin-2 / immunology*
Molecular Sequence Data
Protein Conformation
Stereoisomerism

Substances

Antibodies, Monoclonal
Antigens
Immunoglobulin Fab Fragments
Interleukin-2