Abstract
We cloned and sequenced a cDNA from a library of mouse pituitary AtT-20 cells which are known to cleave an endogenous and various foreign prohormones at dibasic sites. This cDNA encodes a novel 753-residue protein, named PC3, which is structurally related to the yeast Kex2 protease involved in precursor cleavage at dibasic sites and to recently identified mammalian Kex2-like proteins, furin and PC2. Among examined cell lines and tissues, PC3 mRNA was only detected in AtT-20 cells. The substrate specificity of PC3 expressed in mammalian cells was similar to that observed in AtT-20 cells. We conclude that PC3 is a resident prohormone processing endoprotease in AtT-20 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cell Line
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Cloning, Molecular
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DNA / genetics
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Gene Expression Regulation, Enzymologic
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Hormones / metabolism*
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Mice
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Molecular Sequence Data
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Pro-Opiomelanocortin / biosynthesis
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Pro-Opiomelanocortin / genetics*
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Proprotein Convertases
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Receptors, Cell Surface / metabolism
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Serine Endopeptidases / genetics
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Serine Endopeptidases / metabolism*
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Substrate Specificity
Substances
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Hormones
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Receptors, Cell Surface
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Pro-Opiomelanocortin
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DNA
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Proprotein Convertases
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Serine Endopeptidases
Associated data
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GENBANK/M63255
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GENBANK/M64332
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GENBANK/S57661
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GENBANK/S57664
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GENBANK/S64490
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GENBANK/S69601
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GENBANK/S69604
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GENBANK/S69828
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GENBANK/S69830
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GENBANK/X57088