Abstract
To search for genes that can collaborate with myc in lymphomagenesis, we exploited retroviral insertional mutagenesis in E mu-myc transgenic mice. Moloney murine leukemia virus accelerated development of B lymphoid tumors. Three quarters contained a provirus within the known pim-1 or pim-2 loci, new loci bmi-1 and emi-1, or combinations of these. bmi-1 insertions predominated, occurring in half the tumors, and resulted in elevated bmi-1 mRNA levels. Significantly, the bmi-1 gene, which is expressed in diverse normal cells, encodes a Cys/His metal-binding motif (C3HC4) that resembles those in several DNA-binding proteins and defines a new category of zinc finger gene. Thus, myc-induced lymphomagenesis can entail the concerted action of several genes, including the presumptive nuclear regulator bmi-1.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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B-Lymphocytes / immunology
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Base Sequence
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DNA-Binding Proteins / genetics*
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Genes, Immunoglobulin*
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Genes, Regulator
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Genes, myc*
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Immunoglobulin mu-Chains / genetics*
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Lymphoma / genetics*
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Lymphoma / immunology
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Mice
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Mice, Transgenic
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Molecular Sequence Data
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Moloney murine leukemia virus / genetics*
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Multigene Family*
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Nuclear Proteins / genetics*
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Polycomb Repressive Complex 1
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Proteins
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Proto-Oncogene Proteins*
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Proviruses / genetics
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Repressor Proteins*
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Restriction Mapping
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Sequence Homology, Nucleic Acid
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Zinc Fingers / genetics*
Substances
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Bmi1 protein, mouse
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DNA-Binding Proteins
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Emi1 protein, mouse
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Immunoglobulin mu-Chains
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Nuclear Proteins
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Proteins
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Proto-Oncogene Proteins
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Repressor Proteins
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Polycomb Repressive Complex 1
Associated data
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GENBANK/M60958
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GENBANK/M60959
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GENBANK/M60960
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GENBANK/M60961
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GENBANK/M60962
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GENBANK/M64067
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GENBANK/M64068
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GENBANK/M64383
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GENBANK/M64384
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GENBANK/M86183