Abstract
We have amplified, cloned, and sequenced 583 bp GCAP-II/uroguanylin-specific cDNA from human colon cDNA first strand. The cDNA codes for a putative 112 amino-acid precursor protein including the sequence of uroguanylin and GCAP-II. Northern blot hybridization revealed a high level expression of the GCAP-II gene in human colon, but not in the kidney. This expression of GCAP-II indicates a pivotal role in cGMP-mediated functions of the colon.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Calcium-Binding Proteins / genetics*
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Colon / metabolism*
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Cyclic GMP / metabolism
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DNA, Complementary
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Guanylate Cyclase-Activating Proteins
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Humans
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Molecular Sequence Data
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Natriuretic Peptides
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Peptides / genetics*
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Protein Precursors / genetics*
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RNA Precursors / genetics
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Sequence Analysis, DNA
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Tissue Distribution
Substances
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Calcium-Binding Proteins
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DNA, Complementary
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GUCA1B protein, human
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Guanylate Cyclase-Activating Proteins
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Natriuretic Peptides
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Peptides
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Protein Precursors
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RNA Precursors
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RNA, Messenger
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uroguanylin
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Cyclic GMP