Abstract
Five distinct novel transcripts (RES4-22, -23, -24, -25 and -26) that mapped to the 1-Mb interval between D4S180 and D4S183 on human chromosome 4p16.3 close to the Huntington's disease (HD) gene were isolated, and the structure and exon/intron organization of each gene were thoroughly analyzed. The transcripts of the RES4-22, -23 and -24 genes each have several isoforms by alternative splicing and these have also been defined. Two transcripts, RES4-24 and RES4-25, reside in the same genomic region with opposite polarities and they also clearly overlap. Among these transcripts, RES4-26 was found to encode a novel zinc finger protein. The transcript map based upon our current level of analysis combined with data from previous studies reveals the gene-rich nature and the intricate organization of the genes in the HD locus.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Blotting, Northern
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Chromosome Mapping
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Chromosomes, Human, Pair 4*
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Cloning, Molecular
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DNA, Complementary
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Exons / genetics
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Gene Expression
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Humans
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Huntington Disease / genetics*
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Introns / genetics
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Nuclear Proteins
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Analysis, DNA
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Transcription, Genetic*
Substances
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DNA, Complementary
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NOP14 protein, human
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Nerve Tissue Proteins
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Nuclear Proteins
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RNA, Messenger
Associated data
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GENBANK/AB000459
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GENBANK/AB000460
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GENBANK/AB000461
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GENBANK/AB000462
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GENBANK/AB000463
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GENBANK/AB000464
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GENBANK/AB000465
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GENBANK/AB000466
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GENBANK/AB000467
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GENBANK/AB000468
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GENBANK/AB001563