Abstract
Uroguanylin, a member of the guanylin peptide family, is an endogenous activator of intestinal guanylate cyclase (GC-C). A cDNA encoding a precursor for human uroguanylin was cloned from a human colon cDNA library and sequenced. The precursor was 112 amino acids long and included a signal peptide at the N-terminus and the human uroguanylin sequence at the C-terminus. RNA blot analysis and the reverse transcription-polymerase chain reaction (RT-PCR) showed that human uroguanylin mRNA is expressed in the stomach and intestine. Uroguanylin, as well as guanylin, may be a potent physiological regulator of intestinal fluid and electrolyte transport.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA Primers
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DNA, Complementary
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Gastrointestinal Hormones*
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Gene Expression*
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Gene Library
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Humans
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Molecular Sequence Data
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Natriuretic Peptides
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Oligonucleotides, Antisense
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Peptide Biosynthesis*
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Peptides*
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Polymerase Chain Reaction
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Protein Precursors / biosynthesis*
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Protein Sorting Signals / biosynthesis
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RNA, Messenger / biosynthesis
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Sequence Homology, Amino Acid
Substances
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DNA Primers
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DNA, Complementary
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Gastrointestinal Hormones
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Natriuretic Peptides
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Oligonucleotides, Antisense
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Peptides
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Protein Precursors
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Protein Sorting Signals
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RNA, Messenger
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preproguanylin
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guanylin
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uroguanylin