Abstract
Barley Rar1 is a convergence point in the signaling of resistance to powdery mildew, triggered by multiple race-specific resistance (R) genes. Rar1 is shown to function upstream of H2O2 accumulation in attacked host cells, which precedes localized host cell death. We isolated Rar1 by map-based cloning. The sequence of the deduced 25.5 kDa protein reveals two copies of a 60-amino acid domain, CHORD, conserved in tandem organization in protozoa, plants, and metazoa. CHORD defines a novel eukaryotic Zn2+-binding domain. Silencing of the C. elegans CHORD-containing gene, chp, results in semisterility and embryo lethality, suggesting an essential function of the wild-type gene in nematode development. Our findings indicate that plant R genes have recruited a fundamental cellular control element for signaling of disease resistance and cell death.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Amino Acid Sequence
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Animals
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Base Sequence
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / growth & development
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Caenorhabditis elegans / physiology*
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Carrier Proteins / classification
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Carrier Proteins / genetics
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Carrier Proteins / physiology*
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Cell Death
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Cloning, Molecular
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DNA, Complementary
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Drosophila melanogaster / genetics
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Eukaryotic Cells
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Genes, Plant
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Hordeum / genetics
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Hordeum / physiology*
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Humans
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Hydrogen Peroxide / metabolism
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Mutation
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Plant Diseases
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Plant Proteins*
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RNA Splicing
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Sequence Homology, Amino Acid
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Signal Transduction*
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Toxoplasma / genetics
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Transcription, Genetic
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Zinc*
Substances
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Carrier Proteins
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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Plant Proteins
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Rar1 protein, Hordeum vulgare
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Hydrogen Peroxide
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Zinc
Associated data
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GENBANK/AF192261
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GENBANK/AF192262
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GENBANK/AF192263
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GENBANK/AF192264
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GENBANK/AF192465
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GENBANK/AF192466
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GENBANK/AF192467