Abstract
We performed genome wide gene expression analysis on L4 stage Caenorhabditis elegans rrf-3(pk1426) and eri-1(mg366) mutant strains to study the effects caused by loss of their encoded proteins, which are required for the accumulation of endogenous secondary siRNAs. Mutant rrf-3 and eri-1 strains exhibited 72 transcripts that were co-over-expressed and 4 transcripts co-under-expressed (>2-fold, P<0.05) compared to N2 wild type strain. Ontology analysis indicated these transcripts were over represented for protein phosphorylation and sperm function genes. These results provide additional support for the hypothesis that RRF-3 and ERI-1 act together in the endo-siRNA pathway, and furthermore suggests their involvement in additional biological processes.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Caenorhabditis elegans / genetics*
-
Caenorhabditis elegans Proteins / genetics
-
Caenorhabditis elegans Proteins / physiology*
-
Exoribonucleases / genetics
-
Exoribonucleases / physiology*
-
Gene Expression Profiling
-
Gene Expression Regulation*
-
Genes, Helminth*
-
Genome
-
Oligonucleotide Array Sequence Analysis
-
RNA, Small Interfering / metabolism
-
RNA-Dependent RNA Polymerase / genetics
-
RNA-Dependent RNA Polymerase / physiology*
-
Reverse Transcriptase Polymerase Chain Reaction
Substances
-
Caenorhabditis elegans Proteins
-
RNA, Small Interfering
-
RNA-Dependent RNA Polymerase
-
RNA-directed RNA polymerase RRF-3, C elegans
-
ERI-1 protein, C elegans
-
Exoribonucleases