THUMP--a predicted RNA-binding domain shared by 4-thiouridine, pseudouridine synthases and RNA methylases

L Aravind; E V Koonin

doi:10.1016/s0968-0004(01)01826-6

THUMP--a predicted RNA-binding domain shared by 4-thiouridine, pseudouridine synthases and RNA methylases

Trends Biochem Sci. 2001 Apr;26(4):215-7. doi: 10.1016/s0968-0004(01)01826-6.

Authors

L Aravind¹, E V Koonin

Affiliation

¹ National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA. aravind@ncbi.nlm.nih.gov

PMID: 11295541
DOI: 10.1016/s0968-0004(01)01826-6

Abstract

Sequence profile searches were used to identify an ancient domain in ThiI-like thiouridine synthases, conserved RNA methylases, archaeal pseudouridine synthases and several uncharacterized proteins. We predict that this domain is an RNA-binding domain that adopts an alpha/beta fold similar to that found in the C-terminal domain of translation initiation factor 3 and ribosomal protein S8.

MeSH terms

Amino Acid Sequence
Binding Sites
Hydro-Lyases / metabolism*
Molecular Sequence Data
RNA / metabolism*
RNA-Binding Proteins / chemistry
RNA-Binding Proteins / metabolism*
Sequence Homology, Amino Acid
Thiouridine / metabolism*
tRNA Methyltransferases / metabolism*

Substances

RNA-Binding Proteins
Thiouridine
RNA
tRNA Methyltransferases
Hydro-Lyases
pseudouridylate synthetase