Abstract
Two SH3 domain-containing cytosolic components of the NADPH oxidase, p47phox and p40phox, are shown by analyses of their sequences to contain single copies of a novel class of domain, the PX (phox) domain. Homologous domains are demonstrated to be present in the Cpk class of phosphatidylinositol 3-kinase, S. cerevisiae Bem1p, and S. pombe Scd2, and a large family of human sorting nexin 1 (SNX1) homologues. The majority of these domains contains a polyproline motif, typical of SH3 domain-binding proteins. Two further findings are reported. A third NADPH oxidase subunit, p67phox, is shown to contain four tetratricopeptide repeats (TPRs) within its N-terminal RaclGTP-binding region, and a 28 residue motif in p40phox is demonstrated to be present in protein kinase C isoforms iota/lambda and zeta, and in three ZZ domain-containing proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Carrier Proteins / chemistry
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Carrier Proteins / metabolism*
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Humans
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Molecular Sequence Data
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NADPH Oxidases / chemistry*
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Phosphatidylinositol 3-Kinases
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Phosphotransferases (Alcohol Group Acceptor) / chemistry
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Phosphotransferases (Alcohol Group Acceptor) / metabolism*
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Sequence Homology, Amino Acid
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Vesicular Transport Proteins*
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src Homology Domains*
Substances
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Carrier Proteins
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Vesicular Transport Proteins
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NADPH Oxidases
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Phosphatidylinositol 3-Kinases
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Phosphotransferases (Alcohol Group Acceptor)
Associated data
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GENBANK/D11379
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GENBANK/D24276
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GENBANK/D28076
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GENBANK/L11455
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GENBANK/M25665
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GENBANK/N48381
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GENBANK/R21308
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GENBANK/R56645
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GENBANK/U00059
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GENBANK/U12539
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GENBANK/U33007
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GENBANK/U38545
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GENBANK/U51033
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GENBANK/U52192
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GENBANK/U52193
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GENBANK/U53225
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GENBANK/U55854
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GENBANK/X66371
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GENBANK/X77094
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GENBANK/Z28256
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GENBANK/Z28303
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GENBANK/Z36069
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GENBANK/Z44623
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GENBANK/Z48229
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GENBANK/Z48613
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GENBANK/Z50142
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GENBANK/Z69660
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GENBANK/Z74161
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GENBANK/Z74977
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GENBANK/Z75265