Abstract
We have cloned the murine CCR6 receptor and its ligand, the beta-chemokine mMIP-3alpha. Calcium mobilization assays performed with mCCR6 transfectants showed significant responses upon addition of mMIP-3alpha. Murine MIP-3alpha RNA is expressed in thymus, small intestine and colon, whereas mCCR6 RNA is expressed in spleen and lymph nodes. RT-PCR analysis of FACS-sorted lymphoid and antigen presenting cell subsets showed mCCR6 expression mainly in B cells, CD8- splenic dendritic cells and CD4+ T cells. The cloning and functional characterization of the mCCR6 and mMIP-3alpha will allow the study of the role of these proteins in mouse models of inflammation and immunity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigen-Presenting Cells / metabolism
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Calcium Signaling / drug effects
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Cell Line
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Chemokine CCL20
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Chemokines, CC / chemical synthesis
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Chemokines, CC / chemistry
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Chemokines, CC / genetics*
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Chemokines, CC / pharmacology*
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Cloning, Molecular*
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Gene Expression*
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Gene Library
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Humans
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Ligands
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Lymphoid Tissue / metabolism
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Macrophage Inflammatory Proteins*
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Mice
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Molecular Sequence Data
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Organ Specificity
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Protein Sorting Signals
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Receptors, CCR6
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Receptors, Chemokine / chemistry
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Receptors, Chemokine / genetics*
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Receptors, Chemokine / metabolism
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Sequence Alignment
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Transfection
Substances
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CCL20 protein, human
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CCR6 protein, human
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Chemokine CCL20
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Chemokines, CC
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Ligands
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Macrophage Inflammatory Proteins
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Protein Sorting Signals
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RNA, Messenger
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Receptors, CCR6
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Receptors, Chemokine
Associated data
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GENBANK/AJ222694
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GENBANK/AJ222714