Abstract
Telomere maintenance has been proposed as an essential prerequisite to human tumor development. The telomerase enzyme is itself a marker for tumor cells, but the genetic alterations that activate the enzyme during neoplastic transformation have remained a mystery. Here, we show that Myc induces telomerase in both normal human mammary epithelial cells (HMECs) and normal human diploid fibroblasts. Myc increases expression of hEST2 (hTRT/TP2), the limiting subunit of telomerase, and both Myc and hEST2 can extend the life span of HMECs. The ability of Myc to activate telomerase may contribute to its ability to promote tumor formation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Division / physiology
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Cell Line
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DNA-Binding Proteins
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Enzyme Activation / genetics
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Gene Expression / genetics
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Humans
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Proteins / genetics
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Proto-Oncogene Proteins c-myc / genetics*
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Proto-Oncogene Proteins c-myc / physiology*
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RNA*
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RNA, Messenger / metabolism
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Recombinant Proteins / genetics
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Telomerase / genetics*
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Tumor Cells, Cultured / enzymology
Substances
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DNA-Binding Proteins
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Proteins
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Proto-Oncogene Proteins c-myc
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RNA, Messenger
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Recombinant Proteins
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telomerase RNA
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RNA
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TERT protein, human
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Telomerase