Three homologs of rds/peripherin in Xenopus laevis photoreceptors that exhibit covalent and non-covalent interactions

W Kedzierski; W N Moghrabi; A C Allen; M M Jablonski-Stiemke; S M Azarian; D Bok; G H Travis

doi:10.1242/jcs.109.10.2551

Three homologs of rds/peripherin in Xenopus laevis photoreceptors that exhibit covalent and non-covalent interactions

J Cell Sci. 1996 Oct:109 ( Pt 10):2551-60. doi: 10.1242/jcs.109.10.2551.

Authors

W Kedzierski¹, W N Moghrabi, A C Allen, M M Jablonski-Stiemke, S M Azarian, D Bok, G H Travis

Affiliation

¹ Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas 75235-9111, USA.

PMID: 8923216
DOI: 10.1242/jcs.109.10.2551

Abstract

We have isolated and characterized three homologs of mammalian rds/peripherin from Xenopus retinae. One (xrds38) is likely the Xenopus ortholog, while the other two (xrds36 and -35) are more distant relatives. By immunocytochemical analysis of retinal sections, xrds38 is distributed in both rod and cone photoreceptors, while xrds36 and xrds35 are present in rods only. At the EM level, xrds38 is present specifically in the rims and incisures of rod and cone outer segment discs. All are N-glycosylated and form covalent dimers. Immunoprecipitation analysis showed that in rods, these three proteins interact to form heterotetrameric or higher-order complexes. The pattern of sequence conservation among the xrds proteins, mammalian rds/peripherin, and mammalian rom-1 suggest that the central portion of the intradiscal D2 loop contains the interacting structural elements.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Eye Proteins / analysis
Eye Proteins / chemistry*
Eye Proteins / genetics
Eye Proteins / isolation & purification
Fluorescent Antibody Technique, Indirect
Immunohistochemistry
Intermediate Filament Proteins / analysis
Intermediate Filament Proteins / chemistry*
Intermediate Filament Proteins / genetics
Intermediate Filament Proteins / isolation & purification
Membrane Glycoproteins*
Microscopy, Electron
Models, Biological
Molecular Sequence Data
Nerve Tissue Proteins*
Neuropeptides / analysis
Neuropeptides / chemistry*
Neuropeptides / genetics
Neuropeptides / isolation & purification
Peripherins
Photoreceptor Cells / chemistry*
Photoreceptor Cells / ultrastructure
RNA, Messenger / analysis
Sequence Homology, Amino Acid
Xenopus Proteins
Xenopus laevis

Substances

Eye Proteins
Intermediate Filament Proteins
Membrane Glycoproteins
Nerve Tissue Proteins
Neuropeptides
PRPH protein, Xenopus
Peripherins
RNA, Messenger
Xenopus Proteins

Associated data

GENBANK/L79913
GENBANK/L79914
GENBANK/L79915