Abstract
Mitochondrial transcription factor A (mtTFA) is a key regulator of mammalian mitochondrial DNA transcription. We report here that a testis-specific isoform of mouse mtTFA lacks the mitochondrial targeting sequence and is present in the nucleus of spermatocytes and elongating spermatids, thus representing the first reported mammalian gene encoding protein isoforms targeted for the mitochondria or the nucleus. The presence of the mitochondrial transcriptional activator in the nucleus raises the possibility of a role for this protein in both genetic systems. Mutations in the nuclear mtTFA gene may therefore exhibit phenotypic consequences due to altered function in either or both genetic compartments.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Nucleus / chemistry
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Cell Nucleus / genetics
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Cloning, Molecular
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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High Mobility Group Proteins / genetics*
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Male
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Mice
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Mice, Inbred Strains
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Mitochondria / chemistry
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Mitochondrial Proteins*
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Molecular Sequence Data
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Nuclear Proteins*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Spermatocytes / chemistry
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Testis / chemistry*
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Testis / metabolism
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Transcription Factors / biosynthesis
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription, Genetic
Substances
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DNA-Binding Proteins
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High Mobility Group Proteins
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Mitochondrial Proteins
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Nuclear Proteins
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Tfam protein, mouse
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Transcription Factors
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mitochondrial transcription factor A
Associated data
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GENBANK/U57939
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GENBANK/U63858
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GENBANK/U63859
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GENBANK/U63860
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GENBANK/U71384
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GENBANK/U71385
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GENBANK/U71386
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GENBANK/U71387
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GENBANK/U71388
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GENBANK/U71389
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GENBANK/U71390
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GENBANK/U71391
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GENBANK/U71392
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GENBANK/U71393
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GENBANK/U71394
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GENBANK/U71395
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GENBANK/U71396
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GENBANK/U71397