Abstract
We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, A Golgi SNARE of 28 kD, is efficiently packaged into Golgi-derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibody Specificity
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Base Sequence
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Binding, Competitive / physiology
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Biological Transport / physiology
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CHO Cells / chemistry
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CHO Cells / metabolism
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CHO Cells / ultrastructure
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Carrier Proteins / immunology
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Carrier Proteins / metabolism
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Cell Fractionation
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Coated Vesicles / chemistry
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Cricetinae
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Cytoplasm / chemistry
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Golgi Apparatus / chemistry
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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Intracellular Membranes / chemistry
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Membrane Proteins / immunology
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Membrane Proteins / isolation & purification
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Membrane Proteins / metabolism*
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Microscopy, Immunoelectron
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Molecular Sequence Data
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Nerve Tissue Proteins / isolation & purification
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Nerve Tissue Proteins / metabolism
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SNARE Proteins
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Vesicular Transport Proteins*
Substances
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Carrier Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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SNARE Proteins
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Vesicular Transport Proteins