Abstract
We generated interleukin-5 receptor alpha chain (IL-5R alpha)-deficient (IL-5R alpha-/-) mice by gene targeting. The IL-5R alpha-/- mice showed decreased numbers of B-1 cells concomitant with low serum concentrations of IgM and IgG3. They showed no IL-5-induced enhancement of B cell responses to T-independent antigens. The number of alpha beta T cell receptor-positive thymocytes tended to decrease in 3-week-old IL-5R alpha-/- mice, returning to normal by 6 weeks of age. The IL-5R alpha-/- mice produced basal levels of eosinophils, while their bone marrow cells failed to form eosinophilic colonies in response to IL-5. Impaired eosinophilopoiesis in IL-5R alpha-/-mice enhanced the survival of Angiostrongylus cantonensis. These results indicate that IL-5-induced eosinophils serve as potent effector cells in the killing of Angiostrongylus cantonensis in mice.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Angiostrongylus cantonensis / growth & development
-
Angiostrongylus cantonensis / immunology*
-
Animals
-
B-Lymphocytes / immunology
-
B-Lymphocytes / metabolism
-
B-Lymphocytes / pathology*
-
Brain Diseases / genetics
-
Brain Diseases / immunology
-
Brain Diseases / parasitology
-
Cell Differentiation / immunology
-
Eosinophils / pathology
-
Hematopoiesis / immunology
-
IgG Deficiency / genetics
-
Immunoglobulin M / deficiency
-
Interleukin-5 / metabolism*
-
Interleukin-5 / therapeutic use
-
Lymphopenia / genetics
-
Lymphopenia / immunology
-
Mice
-
Mice, Mutant Strains
-
Receptors, Interleukin / deficiency*
-
Receptors, Interleukin / genetics*
-
Receptors, Interleukin / immunology
-
Receptors, Interleukin-5
-
Strongylida Infections / cerebrospinal fluid
-
Strongylida Infections / genetics
-
Strongylida Infections / immunology*
-
T-Lymphocytes / pathology
Substances
-
Immunoglobulin M
-
Interleukin-5
-
Receptors, Interleukin
-
Receptors, Interleukin-5